Ploug Group

All publications - 2001 to present

Lin, L., Gårdsvoll, H., Huai, Q., Huang, M., and Ploug, M.:
Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: Implications for preclinical cancer therapy.
J Biol Chem. 2010; 285: 10982-92.

Morten G. Rasch, Ida K. Lund, Martin Illemann, Gunilla Høyer-Hansen, and Henrik Gårdsvoll:
Purification and characterization of recombinant full-length and protease-domain of murine MMP-9 in Drosophila S2 cells.
Protein Expression and Purification. 2010, 72: 87-94

Alpízar-Alpízar, W., Nielsen, B.S., Sierra, R., Illemann, M., Ramı´rez, J.A., Arias, A., Dura´n, S., Skarstein, A., Ovrebo, K., Lund, L.R. and Laerum, O.D.:
Urokinase plasminogen activator receptor is expressed in invasive cells in gastric carcinomas from high- and low-risk countries.
Int. J. Cancer. 2010, 126: 405–415.

Alpízar-Alpízar, W., Laerum, O.D., Illemann, M., Ramírez, J.A., Arias, A., Malespín-Bendaña, W., Ramírez, V., Lund, L.R., Borregaard, N., Nielsen, B.S.:
Neutrophil gelatinase-associated lipocalin (NGAL/Lcn2) is upregulated in gastric mucosa infected with Helicobacter pylori.
Virchows Arch. 2009, 455:225–233.

Axelsen, T.V., Holm, A., Birkelund, S., Christiansen, G., Ploug, M. and Holm, I.E.:
Specific recognition of the C-terminal end of Aβ42 by a high affinity monoclonal antibody.
Mol. Immunol. 2009, 46: 2267-73

Blouse, G.E., Dupont, D.M., Schar, C.R., Jensen, J.K., Minor, K.H., Anagli, J.Y., Gårdsvoll, H., Ploug, M., Peterson, C.B., and Andreasen, P.A.:
Interactions of plasminogen activator inhibitor-1 with vitronectin involve an extended binding surface and induce mutual conformational rearrangements.
Biochemistry. 2009, 48, 1723-1735.

Lund, I.K., Jögi, A., Rønø, B., Rasch, M.G., Lund, L.R., Almholt, K., Gårdsvoll, H., Behrendt, N., Rømer, J. and Høyer-Hansen, G.:
Antibody-mediated targeting of the uPA proteolytic function. Neutralizes fibrinolysis in vivo.
J.Biol. Chem. 2008, 283: 32506-15.

Bache, N., Rand, K.D., Roepstorff, P., Ploug, M. and Jørgensen, T.J.D.:
Hydrogen Atom Scrambling in Selectively Labeled Anionic Peptides Upon Collisional Activation by MALDI Tandem Time-of-Flight Mass Spectrometry.
J Am Soc Mass Spectrom. 2008, 19:1719-25.

Jacobsen, B. and Ploug, M.:
The urokinase receptor and its structural homologue C4.4A in human cancer: Expression, prognosis and pharmacological inhibition.
Curr Med Chem., 2008, 15: 2559-73.

Li, Z-B., Niu, G., Wang, H., He, L., Yang, L., Ploug, M. and Chen, X.:
Imaging of Urokinase-type Plasminogen Activator Receptor ExpressionUsing a 64Cu-Labeled Linear Peptide Antagonist by microPET.
Clin Cancer Res., 2008, 14:4758-66.

Hillig, T., Engelholm, L.H., Ingvarsen, S., Madsen, D.H., Gårdsvoll, H., Larsen, J.K., Ploug, M., Danø, K., Kjøller, L. and Behrendt, N.:
A composite role of vitronectin and urokinase in the modulation of cell morphology upon expression of the urokinase receptor.
J.Biol.Chem. 2008, 283: 15217-15223.

Green, K.A., Almholt, K.A., Ploug, M., Rønø, B., Johnsen, M., Castellino, F.J., Bugge, T.H., Rømer, J. and Lund, L.R.:
Pro-fibrinolytic effects of metalloproteinases during skin wound healing in the absence of plasminogen.
J. Invest. Dermatol. 2008, 128: 2092-2101.

Kjaergaard, M., Hansen, L.V., Jacobsen, B., Gårdsvoll, H. and Ploug, M.:
Structure and ligand interactions of the urokinase receptor (uPAR).
Frontiers in Bioscience, 2008, 13: 5441-5461.

Hansen, L.V., Lærum, O.D., Illemann, M., Nielsen, B.S., and Ploug, M.:
"Altered expression of the urokinase receptor homologue, C4.4A, in invasive areas of human esophageal squamous cell carcinoma."
Int. J. Cancer, 2008, 122: 734-741

Jacobsen, B., Kjaergaard, M., Gårdsvoll, H., and Ploug, M.:
Structure and inhibition of the urokinase-type plasminogen activator receptor (uPAR).
In “The Cancer Degradome-Proteases and Cancer Biology”. Springer Verlag. 2007

Madsen, D.H., Engelholm, L.H., Ingvarsen, S., Hillig, T., Wagenaar-Miller, R.A., Kjøller, L., Gårdsvoll, H., Høyer-Hansen, G., Holmbeck, K., Bugge, T.H., Behrendt, N.:
Extracellular collagenases and the endocytic receptor, uPARAP/Endo180, cooperate in fibroblast-mediated collagen degradation.
J. Biol. Chem. 2007, 282:27037-27045.

Hansen, L.V., Skov, B. G., Ploug, M., and Pappot, H.:
"Tumor cell Expression of C4.4A, a structural homologue of the urokinase receptor, is correlated with poor prognosis in non-small cell lung cancer." Lung Cancer. 2007, 58: 260-266

Kjaergaard, M., Gårdsvoll, H., Hirschberg, D., Nielbo, S., Mayasundari, A., Peterson, C., Jansson, A., Jørgensen, T.J.D., Poulsen, F. M., and Ploug, M.:
"Solution structure of recombinant somatomedin B domain from vitronectin produced in Pichia pastoris."
Protein Science. 2007, 16: 1934-1945

Pass, J., Jögi, A., Lund, I.L., Rønø, B., Rasch, M.G., Gårdsvoll, H., Lund, L.R., Ploug, M., Rømer, J., Danø, K., and Høyer-Hansen, G.:
"Murine monoclonal antibodies against murine uPA receptor produced in gene-deficient mice: Inhibitory effects on receptor-mediated uPA activity in vitro and in vivo."
Thrombosis and Haemostasis. 2007, 6: 1013-1022

Gårdsvoll, H and Ploug, M.:
"Mapping of the vitronectin binding site on the urokinase receptor. Involvement of a coherent receptor interface comprising residues from both domain I and the flanking interdomain linker region."
J. Biol. Chem., 2007, 282: 13561-13572

Gårdsvoll, H., Hansen, L.V., Jørgensen, T.J.D, and Ploug, M.:
"A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor." Protein Purification and Expression. 2007, 52: 384-394

Jacobsen, B., Gårdsvoll, H., Funch, G.J., Østergård, S., Barkholt, V., and Ploug, M.:
"One-step affinity purification of recombinant urokinase-type plasminogen activator receptor using a synthetic peptide developed by combinatorial chemistry."
Protein Purification and Expression. 2007, 52: 286-296

Gårdsvoll, H., Gilquin, B., LeDu, M.H., Ménez, A., Jørgensen, T.D.J. and Ploug, M.:
"Charaterization of the functional epitope on the urokinase receptor. Complete alanine scanning mutagenesis supplemented by chemical crosslinking."
J. Biol. Chem., 2006, 281: 19260-19272

Lund, LR., Green, K.A., Stoop, A.A., Ploug, M., Almholt, K., Lilla, J., Nielsen, B.S., Christensen, I.J., Craik, C.S., Werb, Z, Danø, K., and Rømer, J.:
"Plasminogen activation independent of uPA and tPA maintains wound healing in gene-deficient mice."
EMBO J., 2006, 25: 2686-2697

Chaurasia, P., Aguirre-Ghiso, J.A., Liang, O.D., Gårdsvoll, H., Ploug, M and Ossowski, L.:
"A region in urokinase plasminogen receptor domain III controlling a functional association with a5b1ntegrin and tumor growth."-i
J. Biol. Chem., 2006, 281: 14852-14863

Jørgensen, T.J., Bache, N., Gårdsvoll, H., Roepstorff, P. and Ploug, M.:
"Collisional activation by MALDI tandem time-of-flight mass spectrometry induces intramolecular migration of amide hydrogens in protonated peptides."
Mol. Cell. Proteomics. 2005, 4: 1910-1919

Llinas, P., Le Du, M., Gårdsvoll, H., Danø, K., Ploug, M., Gilquin, B., Stura, E.A., and Ménez, A.:
"Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide."
EMBO J., 205, 24: 1655-1663

Danø, K., Behrendt, N., Høyer-Hansen, G., Johnsen, M., Lund, L.R., Ploug, M., and Rømer, J.:
"Plasminogen activation and cancer."
Thromb. Haemost. 2005, 93: 676-681

Jørgensen, T. J. D., Gårdsvoll, H., Ploug, M., and Roepstorff, P.:
"Intramolecular migration of amide hydrogens in protonated peptides during collision induced activation."
J. Am. Chem. Soc., 2005, 127: 2785-2793

Piironen, T., Laursen, B., List, K., Gårdsvoll, H., Pass, J., Ploug, M., Danø, K., and Høyer-Hansen, G.:
"Design of specific immunoassays for the detection of intact and cleaved forms of the urokinase receptor."
Clin. Chem., 2004, 50: 2059-2068

Jørgensen, T.J.D., Gårdsvoll, H., Danø, K., Roepstorff, P. and Ploug, M.:
"Dynamics of urokinase receptor interaction with peptide antagonists studied by amide-hydrogen exchange and mass spectrometry."
Biochemistry. 2004, 43: 15044-15057

Hansen, L.V., Gårdsvoll, H., Nielsen, B. S., Lund, L. R., Danø, K., Jensen, O. N., and Ploug, M.:
"Structural analysis and tissue localization of human C4.4A: a protein homologue of the urokinase receptor."
Biochem. J. 2004, 380: 845-857

Rømer, J., Nielsen B. S., and Ploug, M.:
"The urokinase receptor as a potential target in cancer therapy."
Current Pharmaceutical Design. 2004, 10: 2359-2376

Gårdsvoll, H; Werner, F. Søndergaard, L., Danø, K., and Ploug, M.:
"Characterization of low-glycosylated forms of soluble human urokinase receptor expressed in Drosophila Schneider S2 cells after deletion of glycosylation sites."
Protein Purification and Expression. 2004, 34: 284-295

Ploug, M.:
"Structure-function relationships in the interaction between the urokinase-type plasminogen activator and its receptor."
Current Pharmaceutical Design. 2003, 9: 1499-1528

Ploug, M., Gårdsvoll, H., Jørgensen, T. D. J., Hansen, L. L., and Danø, K.:
"Structural analysis of the interaction between urokinase and its receptor: a potential target for antinvasive cancer therapy."-i
Biochem. Soc. Trans. 2002, 30: 177-183

Ploug, M., Østergaard, S., Gårdsvoll, H., Kovalski, K., Holst-Hansen, C., Holm, A., Ossowski, L., and Danø, K.:
"Peptide derived antagonists of the urokinase receptor. Affinity maturation by combinatorial chemistry, identification of functional epitopes, and inhibitory effects on cancer cell intravasation."
Biochemistry. 2001, 40: 12157-12168

Solberg, H., Ploug, M., Høyer-Hansen, G., Nielsen, B. S., and Lund, L. R.:
"The murine receptor for urokinase type plasminogen activator is expressed in tissues actively undergoing remodeling."
J. Histochem. Cytochem 2001, 49: 237-246