Henrik Gårdsvoll - Recent Publications

Kriegbaum, M., Persson, M., Haldager, L., Alpizar-Alpizar, W., Jacobsen, B., Gårdsvoll, H., Kjær, A. and Ploug, M.:
Rational targeting of the urokinase receptor (uPAR): Development of inhibitors and nonnvasive imaging probes.-i
Current Drug Targets. In press.

Gårdsvoll, H., Jacobsen, B., Kriegbaum, M., Behrendt, N., Engelholm, L., Østergård, S. and Ploug, M.:
Conformational regulation of urokinase receptor (uPAR) function. Impact of receptor occupancy and of epitope-mapped monoclonal antibodies on lamellipodia induction.
Journal of Biological chemistry. Under revision.

Connolly, B.M., Choi, E.Y., Gårdsvoll, H., Bey, A.L., Currie, B.M., Chavakis, T., Liu, S., Molinolo, A., Ploug, M., Leppla, S.H., and Bugge, T.H.:
Selective abrogation of uPA-uPAR interaction in vivo reveals a novel role in suppression of fibrin-associated inflammation.
Blood, 2010; 116:1593-1603.

Lin, L., Gårdsvoll, H., Huai, Q., Huang, M., and Ploug, M.:
Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: Implications for preclinical cancer therapy.
Journal of Biological chemistry. 2010; 285: 10982-92.

Morten G. Rasch, Ida K. Lund, Martin Illemann, Gunilla Høyer-Hansen, and Henrik Gårdsvoll:
Purification and characterization of recombinant full-length and protease-domain of murine MMP-9 in Drosophila S2 cells.
Protein Expression and Purification. 2010. 72: 87-94.

Blouse, G.E., Dupont, D.M., Schar, C:R:, Jensen, J.K., Minor, K.H., Anagli, J.Y., Gårdsvoll, H., Ploug, M., Peterson, C.B., and Andreasen, P.A.:
"Interactions of plasminogen activator inhibitor-1 with vitronectin involve an extended binding surface and induce mutual conformational rearrangements."
Biochemistry. 2009, 48:1723-1735.

Hillig, T., L. H. Engelholm, S. Ingvarsen, D. H. Madsen, H. Gårdsvoll, J. K. Larsen, M. Ploug, K. Danø, L. Kjøller, and N. Behrendt.:
"A composite role of vitronectin and urokinase in the modulation of cell morphology upon expression of the urokinase receptor."
Journal of Biological Chemistry, 2008, 283: 15217-15223.

Lund, I.K., Jögi, A., Rønø, B., Rasch, M.G., Lund, L.R., Almholt, K., Gårdsvoll, H., Behrendt, N., Rømer, J. and Høyer-Hansen, G.:
"Antibody-mediated targeting of the uPA proteolytic function. Neutralizes fibrinolysis in vivo."
Journal of Biological chemistry. 2008, 283: 32506-15.

Kjaergaard, M., L. V. Hansen, B. Jakobsen, H. Gårdsvoll, and M. Ploug:
"Structure and ligand interactions of the urokinase-type plasminogen activator receptor (uPAR)."
Frontiers in Biosciences, 2008, 13: 5441-546.

Jakobsen, B, M. Kjaergaard, H. Gårdsvoll, and M. Ploug:
"Structure and inhibition of the urokinase-type plasminogen activator receptor (uPAR)."
The Cancer Degradome-Proteases and Cancer Biology, D. Edwards, G. Høyer-Hansen, F. Blasi and B.F. Sloane (eds), Springer Verlag, 2007.

Kjaergaard, M., H. Gårdsvoll, D. Hirschberg, S. Nielbo, A. Mayasundari, C. B. Peterson, A. Jansson, T. J. D. Jørgensen, F. M. Poulsen, and M. Ploug:
"Solution structure of recombinant somatomedin B domain from vitronectin produced in Pichia pastoris."
Protein Science, 2007, 16: 1934-1945.

Madsen, D. H., L. H. Engelholm, S. Ingvarsen, T. Hillig, R. A. Wagenaar-Miller, L. Kjøller, H. Gårdsvoll, G. Høyer-Hansen, K. Holmbeck, T. H. Bugge, and N. Behrendt:
"Extracellular collagenases and the endocytic receptor, uPARAP/Endo180, cooperate in fibroblast-mediated collagen degradation."
Journal of Biological Chemistry, 2007, 282: 27037-27045.

Gårdsvoll, H.. and M. Ploug:
"Mapping of the vitronectin binding site on the urokinase receptor. Involvement of a composite interface of domain I and the flanking interdomain linker region."
Journal of Biological Chemistry, 2007, 282: 13561-13572.

Pass, J., A. Jögi, I.K. Lund, B. Rønø, M.G. Rasch, H. Gårdsvoll, L.R. Lund, M. Ploug, J. Rømer, K. Danø, and G. Høyer-Hansen:
"Murine monoclonal antibodies against murine uPA receptor produced in gene-deficient mice: Inhibitory effects on receptor-mediated uPA activity in vitro and in vivo."
Thrombosis and Haemostasis, 2007, 97: 1013-1022.

Gårdsvoll, H., L.V. Hansen, T. J. D. Jørgensen, and M. Ploug:
"A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor."
Protein Expression and Purification, 2007, 52: 384-394.

Jakobsen, B., H. Gårdsvoll G. J. Funch, S. Østergaard, V. Barkholt, and M. Ploug:
"One-step affinity purification of recombinant urokinase-type plasminogen activator receptor using a synthetic peptide developed by combinatorial chemistry."
Protein Expression and Purification, 2007, 52: 286-296.

Gårdsvoll, H.., B. Gilquin, M. H. Le Du, A. Menez, T. J. D. Jørgensen, and M. Ploug:
"Characterization of the functional epitope on uPAR for uPA. Complete alanine scanning mutagenesis of uPAR supplemented by chemical crosslinking."
Journal of Biological Chemistry, 2006, 281: 19260-19272.

Chaurasia, P., J.A. Aguirre-Ghiso, O.D. Liang, H. Gårdsvoll, M. Ploug, and L. Ossowski:
"A region in uPAR domain III controlling a functional association with 1 - integrin and tumor growth."
Journal of Biological Chemistry, 2006, 281:14852-14863.

Jørgensen, T.J.D., N. Backe, P. Roepstorff, H. Gårdsvoll, and M. Ploug:
"Collisional activation by MALDI tandem time-of-flight mass spectrometry induces intramolecular migration of amide hydrogens in protonated peptides."
Molecular and Cellular Proteomics, 2005, 4: 1910-1919.

Llinas, P., M. H. Le Du, H. Gårdsvoll K. Danø, M. Ploug, B. Gilquin, E. A. Stura and A. Menez:
"Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide."
The EMBO journal, 2005, 24: 1655-1663.

Jørgensen, T.J.D., H. Gårdsvoll M. Ploug and P. Roepstorff:
"Intramolecular migration of amide hydrogens in protonated peptides upon collisional activation."
Journal of American and Chemical Society, 2005, 127: 2785-2793.

Jørgensen, T.J.D.,H. Gårdsvoll, K. Danø, P. Roepstorff and M. Ploug:
"Dynamics of urokinase receptor interaction with peptide antagonists studied by amide-hydrogen exchange and mass spectrometry."
Biochemistry, 2004, 43: 15044-15057.

Piironen, T., B. Laursen, J. Pass, K. List, H. Gårdsvoll, M. Ploug, K. Danø and G. Høyer-Hansen:
"Specific immunoassays for detection of intact and cleaved forms of the urokinase receptor."
Clinical Chemistry, 2004, 50: 2059-2068.

Hansen, L.V., H. Gårdsvoll, B.S. Nielsen, L.L. Lund, K. Danø, O.N. Jensen and M. Ploug:
"Structural analysis and tissue localization of human C4.4A: a protein homoloque of the urokinase receptor."
Biochemical Journal, 2004, 380: 845-857.

Gårdsvoll, H., F. Werner, L. Søndergaard, K. Danø, and M. Ploug:
"Characterization of low-glycosylated forms of soluble human urokinase receptor expressed in Drosophila Schneider 2 cells after deletion of glycosylation-sites."
Protein Expression and Purification, 2004, 34: 284-295.

Pass, J., H. Gårdsvoll L.L. Lund, K. Danø and G. Høyer-Hansen:
"Generation of antibodies to the urokinase receptor (uPAR) by DNA immunization of uPAR knock-out mice: Membrane bound uPAR is not required for an antibody response."
Scand. J. Immunol., 2003, 58: 298-305.

Ploug, M., H. Gårdsvoll T.J. Jørgensen, L. L. Hansen and K. Danø:
"Structural analysis of the interaction between urokinase-type plasminogen activator and its receptor: a potential target for anti - invasive cancer therapy."
Biochem. Soc. Trans., , 2002, 30: 177-183.

Ploug, M., S. Østergaard, H. Gårdsvoll, K. Kovalski, C. Holst-Hansen, A. Holm, L. Ossowski and K. Danø:
"Peptide derived antagonists of the urokinase receptor. Affinity maturation by combinatorial chemistry, identification of functional epitopes, and inhibitory effect on cancer cell intravasation."
Biochemistry, 2001, 40: 12157-12168.